Clinical and aesthetic dermatology thrive because dermatologists meticulously pay attention to every detail. In order to deliver exceptional results to clients, finding the right tools and treatments is akin to discovering a hidden treasure room. What if we told you that there is a key that unlocks the door to youthful, radiant skin and luscious hair—a key forged through meticulous science and unwavering dedication? This key is exosomes, and Advancexo provides it with unparalleled expertise and precision.
Advancexo combines precision, innovation, and quality to produce exosomes that revolutionize skin and hair care. For dermatologists looking to offer their clients unparalleled results, these exosomes are the golden ticket to transforming outcomes and elevating their practice to new heights.
But have you ever wondered how they are made? This is exactly what this blog post will help you understand. We will understand what the exosome manufacturing process is and what makes us a leading exosome manufacturing company in India.
5 Stages of Exosome Manufacturing
In order to produce the highest-quality exosomes, the exosome manufacturing company in India must have an unwavering commitment to quality. Producing exosomes of exceptional quality and purity requires meticulous attention to detail. This means that the process must be validated, carried out by experts in the field, and adhere to the regulatory standards.
As a leading exosome manufacturing company in India, Advancexo’s commitment to quality is reflected at every stage of the exosome manufacturing process.
Step 1: Donor Selection
The first step to harvesting the best exosomes for dermatological use requires us to choose a potent source rich in revitalizing biomolecules. At Advancexo, we have chosen the Whaton’s jelly mesenchymal stem cells obtained from the umbilical cord. We carefully select the umbilical cord donor before we bring them in vitro for exosome extraction.
We carefully take notes on the donor’s medical history to ensure there are no genetic disorders or risks of developing cancer. This is a vital step to ensure the purity of exosomes. After the medical history, we ensure that the donor is in good health to donate the umbilical cord and does not have any infection.
Once the donor’s medical history meets the regulatory standards, we proceed to explain to the donor what we will be using the placenta for and ask for their consent. Once the mother understands and agrees to the use of the umbilical cord tissue, doctors provide it to us after the successful delivery of the newborn baby.
Step 2: Expanding the cells in laboratory conditions
The umbilical cord from the hospital is transported directly to our GMP-complaint facility for further processing. At our facility, the placenta is received by the technical team to begin bringing the tissue into vitro.
The first step is to ensure that there is no contamination, and to ensure this, we start by excising the ends of the umbilical cord. After this step, multiple screening tests are performed to guarantee the absence of bacterial, fungal, mycoplasma, and viral contaminants.
Additionally, any external influence that can determine the fate of the cells is removed from the culture. An unwavering commitment to keep the stem cells free of contaminants is a crucial step that helps Advancexo stand out as a leading manufacturer of exosomes in India.
Then, the extracted stem cells are transferred to the cell culture flask and kept in an incubator to expand. Once the cells start to replicate, a small amount of the stem cells are removed and once again checked for any form of contamination, genetic abnormalities, or infectious diseases. We perform these tests regularly to ensure that the cells maintain their stemness.
It is a double-layered check done to ensure the high-quality exosomes only come from undifferentiated stem cells that are free from any type of abnormality.
Step 3: Extraction of Exosomes
The next stage is where we isolate our magic molecules—exosomes! We collect the medium in which the stem cells are growing and start to process them. As the first step, the collected culture medium is centrifuged to ensure that it is devoid of any living cells or cell debris. Cell debris is removed to ensure that it does not act as an antigen or irritant when applied to a target area of treatment.
The next step is where we get a pure concentration of exosomes. We use tangential flow filtration (TFF) technology to ensure that the exosomes within the size range (30–150 nm) are selected. Employing TFF ensures the highest yield and purity of exosomes, which are free from contaminants that could compromise their safety and efficacy.
Step 4: Sterility and Safety of Exosomes
After collecting a pure concentrate of exosomes, we have to ensure that they are free of any contaminants. Yes, we did check them for abnormalities in the stem cells and other contaminants, but here we characterize the isolated exosomes.
The exosomes are tested for markers of mesenchymal stem cell-derived exosomes such as CD63, CD9, and CD81. Once the surface markers are confirmed, they are checked for their morphology, size, and quantity. If the isolated exosomes meet industrial standards, they are ready to be further processed.
Please note: The exosomes are also tested for endotoxins and treated using a proprietary procedure to sterilize them. It is used to ensure biosafety and includes steps like treatment with virucidal agents and physical means like filtration.
Step 5: Storage of Exosomes
The final step includes storing the exosomes for long term storage and maintaining their stability. This is a crucial step that sets us apart as a leading exosome manufacturing company in India. Once we confirm the required number of exosomes (5 billion for the Rejuve Kit and 10 billion for the Hair Rejuve Kit), we use the lyophilization method to vacuum dry the exosomes.
In this state, these exosomes are immediately packaged. The packaging also includes a bioactivating solution, which is a potent mix of essential nutrients required for skin or hair growth. The Advancexo lyophilized exosomes are stable for 1 year from the date of manufacturing.
At this stage, you can order a skin or hair rejuvenation kit for your client, and we will ship it to you. For using the product, all you have to do is mix the supplied bioactivating solution with the lyophilized exosomes, and they are ready for your client’s treatment.
Key considerations for selecting a reliable exosome manufacturing company in India
Before you integrate exosomes into your practice, it is essential that you weigh several critical factors. These considerations are to be made to ensure that you are able to offer your clients the results they desire with the minimum discomfort. And when it comes to delivering results to your clients, there can be no compromise in the quality of the exosomes.
Firstly, ensure that the exosome manufacturing company in India is committed to delivering quality. A reputable manufacturer will operate under the Good Manufacturing Practices (GMP) framework. It will ensure that the product that you are procuring delivers zero or minimal variations and high efficacy.
Secondly, purity and delivering the promised concentration of exosomes are crucial in helping you deliver visible results to your clientele. A high-quality product will be devoid of any contaminants, and it must be proven with a certificate of analysis. The certificate must mention the concentration of exosomes you are receiving and report whether they are tested for endotoxin presence, among other contaminants.
Thirdly, ask to see the in-house facility of the manufacturer, making sure that the exosome manufacturing company in India is indeed a manufacturer and not a reseller. It also provides proof of the dedication of the manufacturer that there will be continuous improvement of the exosome production process.
Collaborate with the leading manufacturers of exosomes in India.
Advancexo, with its commitment to world standard quality, has positioned itself in a perfect spot to meet this requirement. We are not just an exosome manufacturing company in India; we are pioneers in delivering high quality exosomes to enhance your client care. Our team of experts is ready to offer assistance to you and answer all your questions regarding the Advancexo Skin and Hair Rejuve Kits.
When you choose to work with Advancexo, you are basically making an investment in the continued success of your practice. For us, our exosomes are more than just products; they are a symbol of our unyielding commitment to providing perfection to your clientele.
Final Words
The path to having a satisfied clientele begins with high-quality products that you apply to your clients. Advancexo has perfected the exosome manufacturing process to deliver exosomes of the highest quality, purity, and sterility. By incorporating Advancexo into your practice, you are leveraging the power of natural healing to revolutionize your clientele’s lives. Together, we can mold the future of dermatology.
FAQs
Exosome therapy is increasingly gaining popularity among dermatologists and the general population. It is because mesenchymal stem cell-derived exosomes have the same rejuvenating properties as their parents. Especially, the exosomes isolated from the umbilical cord have crucial growth factors and bioactive molecules that deliver similar benefits as stem cells. All the rejuvenating factors are delivered without the presence of live cells, contrary to stem cell therapy, which uses live cells and is subject to strict regulatory standards.
Exosomes are isolated from live cells, and Advancexo extracts them from umbilical cord derived mesenchymal stem cells. The umbilical cords are obtained at the time of childbirth from informed donors and processed in a sterile environment. From the placenta, we isolate stem cells and then ensure that they stay in their undifferentiated state and produce exosomes with the same rejuvenating factors.
Advancexo’s exosomes are harvested in a sterile GMP-compliant facility. The exosomes are harvested from the in vitro growing stem cells that secrete exosomes in the cell culture medium.
Good Manufacturing Practices (GMP) are the most stringent set of guidelines established by medical regulatory bodies such as the WHO. These guidelines are used to govern the production of biological products, such as exosomes. By following the laid down GMP guidelines, an exosome manufacturing company in India can produce high-quality products that meet regulatory requirements and ensure clients’ safety.
Exosomes are all the same. They are nano-sized vesicles that carry several bioactive molecules with them. The only difference that comes is from the source of exosomes. There are three sources of exosomes for clinical applications. They are either isolated from plants, adipose tissue derived mesenchymal stem cells, or Wharton jelly mesenchymal stem cells. Each one of them has a different composition and delivers different targeted benefits.
